Categories
BLOG

quick blunting kit

Quick blunting kit

For all questions regarding availability please call: 0800/BIOLABS (2465227 / german customers) or 00800/24652277 (austrian customers)

Downloads
  • Pricelist
  • Video Library
  • Catalogs & Literature
  • Press Releases
  • iPhone App
  • Android App
  • ISO Certifications
NEB GmbH
  • Contact
  • About NEB
  • FAQ / Help
  • Imprint
  • Privacy Policy
  • GTC
International
  • International Orders
  • NEB Inc.
Contact

New England Biolabs GmbH
Brüningstr. 50; Geb. B852
D-65926 Frankfurt am Main

Quick Blunting Kit, Article Number: [email protected]

Quick blunting kit

You have no items in your shopping cart.

  • My Account
  • My Wishlist
  • My Cart
  • Checkout
  • Log In
  • Home
  • Products
    • PCR Products
      • Standard PCR
      • Hot-Start PCR
      • Multiplex PCR
      • High-Fidelity & Long-Range PCR
      • Reverse Transcription & RT-PCR
    • Lyophilized PCR Kits
    • Real Time PCR
      • CAPITAL qPCR Mixes
      • CAPITAL One Step qRT-PCR
    • Nucleotides
    • Nucleic Acid Purification
      • DNA Cleanup
      • Plasmid Isolation
      • Total DNA Isolation
      • Total RNA Isolation
      • Virus Nucleic Acid Isolation
      • Deparaffinization
    • Electrophoresis Ladders
      • DNA Electrophoresis
      • Protein Electrophoresis
    • Enzymes and Proteins
      • Polymerases
      • Ligases & NA Modifying Enzymes
      • Inhibitory Antibodies & Proteins
    • Protein Research
      • His-tag Protein Binding
    • Cell-free Protein Synthesis
      • Rapid Translation Systems (RTS)
        • E. coli High Yield Kits
        • E. coli Disulfide and Fab Kits
        • Wheat Germ Kits
        • Insect Membrane Kit
        • Supplements and Accessories
        • Vectors and Linear Template Kits
  • Applications
    • Molecular Biology
      • Standard PCR
      • Hot Start PCR
      • High Fidelity, Long and GC Rich PCR
      • Reverse Transcription and RT PCR
      • Real Time PCR & RT-QPCR
      • Cloning related applications
      • DNA Electrophoresis
      • DNA/RNA Isolation
    • Protein Research
      • Protein Electrophoresis
    • Cell-free Protein Synthesis
      • Cell-free Kits – Overview
  • mAb Services
    • Monoclonal Antibodies
  • OEM
  • Support
    • Technical Support
      • Product Selection Guides
      • Endpoint PCR Technical Guide
      • Endpoint PCR Troubleshooting
      • QPCR Troubleshooting
    • Documentation
  • About
    • Management Team
    • History and Events
      • ISO 13485 & 9001 – 2016
      • Cell-free Synthesis – 2015
      • Arab Lab – 2014
      • Medica – 2013
      • Biotechnica – 2013
      • Bio Malaysia – 2013
      • Portfolio extension – 2013
      • biotechrabbit – 2011
    • Culture and Careers
  • Contact Us
    • Contact biotechrabbit
    • Distribution Partners
    • Request a Sample
  • Home »
  • DNA Blunting Kit

biotechrabbit™ DNA Blunting Kit shows excellent performance in blunting and phosphorylation of double-stranded DNA. The kit is compatible with a wide variety of dsDNA templates such as restriction enzyme digested DNA fragments, plasmid DNA, PCR products with dA-overhang, sheared or nebulized DNA. The kit is optimized for rapid blunting reaction.

Blunting Enzyme Mix

20× Blunting Enzyme Mix, in storage buffer containing 50% (v/v) glycerol.

Optimized 10× Blunting Buffer for rapid blunting reaction.

dNTP Mix containing 10 mM dATP, dCTP, dGTP, dTTP in water.

-20°C (until expiry date – see product label)

Quality Control
Functional Assay

Purified double digested sticky-end DNA is blunted, ligated and successful ligation is confirmed.

Exonuclease Activity

Linearized lambda/HindIII DNA fragments are incubated with the enzyme in a 50 µl reaction mixture for 4 h at 37°C. No DNA degradation observed.

Endonuclease/Nick Activity

Supercoiled plasmid DNA is incubated with the enzyme in a 50 µl reaction mixture for 4 h at 37°C. No conversion of covalently closed circular DNA to nicked DNA detected.

Contamination with E. coli DNA

Absence of E. coli genomic DNA is confirmed by qPCR using a sample of the enzyme and specific primers targeting the E. coli 16S rRNA gene. No contamination detected.

<p>biotechrabbit&trade; DNA Blunting Kit shows excellent performance in blunting and phosphorylation of double-stranded DNA. The kit is compatible with a wide variety of dsDNA templates such as restriction enzyme digested DNA fragments, plasmid DNA, PCR p